Proteome approaches to characterize seed storage proteins related to ditelocentric chromosomes in common wheat (Triticum aestivum L.)

PROTEOMICS ◽  
2002 ◽  
Vol 2 (9) ◽  
pp. 1146-1155 ◽  
Author(s):  
Nazrul Islam ◽  
Sun-Hee Woo ◽  
Hisashi Tsujimoto ◽  
Hiroshi Kawasaki ◽  
Hisashi Hirano
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L

Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

2011 ◽  
Vol 9 (2) ◽  
pp. 256-259
Author(s):  
Zuzana Šramková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Ernest Šturdík
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

Homology of chromosomes of Triticum macha Dek. et Men. and Triticum aestivum L. as shown with the help of genetic markers

Genome ◽  
1990 ◽  
Vol 33 (5) ◽  
pp. 755-757 ◽  
Author(s):  
E. V. Metakovsky ◽  
Z. A. Iakobashvili
Keyword(s):  
Triticum Aestivum ◽  
Genetic Analysis ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Close Relationship ◽  
Homology Relationship ◽  
Relationship Of ◽  
Triticum Macha

Inheritance of the storage protein (gliadin and glutenin) genes of Triticum macha Dek. et Men. and their allelism to Triticum aestivum L. genes have been studied. A close homology of at least chromosomes 1A and 1B of the two species has been found. Results confirm a very close relationship between T. macha and T. aestivum.Key words: seed storage proteins, genetic analysis, chromosome homology, relationship of Triticum macha Dek. et Men. and Triticum aestivum L.

Genetic diversification of the Chinese wheat landrace Mazhamai as revealed by morphological characteristics, seed storage proteins, and microsatellite markers

2007 ◽  
Vol 87 (4) ◽  
pp. 763-771 ◽  
Author(s):  
Lingli Zhang ◽  
Hongjie Li ◽  
Hui Wang ◽  
Lihui Li
Keyword(s):  
Microsatellite Markers ◽  
Storage Proteins ◽  
Genetic Relationships ◽  
Morphological Characteristics ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Geographical Regions ◽  
Wheat Landraces ◽  
Agronomical Traits

Chinese landraces of wheat (Triticum aestivum L. em Thell.) have had significant contributions not only to wheat production, but also to wheat improvement. Mazhamai is one of the most distinctive landraces, and has been used as a foundation parent in developing many outstanding improved cultivars in China. The objective of this study was to determine the genetic relationships of eight wheat landraces with the name Mazhamai, collected from wheat-producing regions with various geographical and microgeographic environments using morphological and agronomical traits, the composition of their gliadins and high-molecular-weight glutenin subunits (HMW-GS) and microsatellite markers. These homonymous accessions of Mazhamai differed, to some extent in their morphological and agronomical traits, gliadin composition, and microsatellite markers, but not for the HMW-GS and similar profiles for 52 of the SSR markers. This suggests that genetic variation must have occurred during the long-term growing of the Mazhamai accessions in various geographical regions. Furthermore, microsatellite analysis also revealed that the B genomewas more variable than the A and D genomes and chromosomes 2A and 2B were the most variable chromosomes among the Mazhamai accessions. However, chromosomes 1A, 1D, 2D, 5B, and 7A were conserved for the SSR loci used in the study. Key words: Wheat landraces, Mazhamai, genetic relationship, gliadin, glutenin, microsatellites

scholarly journals Studying common wheat material from crosses with Aegilops biuncialis vis. using markers for chromosome 1U

2019 ◽  
Vol 25 ◽  
pp. 55-59
Author(s):  
N. A. Kozub ◽  
I. A. Sozinov ◽  
H. Ya. Bidnyk ◽  
N. A. Demianova ◽  
O. I. Sozinova ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Common Wheat ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Bread Making ◽  
Wheat Gene ◽  
The Common ◽  
Gli 1

Aim. The aim of the research was to study common wheat material developed from crosses with Aegilops biuncialis Vis. using storage protein loci as markers for chromosome 1U. Methods. SDS and APAG electrophoreses of seed storage proteins were employed to identify alleles at the Glu-1 and Gli-1 loci. The following markers of chromosome 1U of Ae. biuncialis were used: the Glu-U1 locus encoding high-molecular-weight glutenin subunits located on the long arm (1UL) and the gliadin locus Gli-U1 on the short arm (1US). Results. In F6–F7, elimination of chromosome 1U material with a frequency of about 0.222 proceeded. This indicates selection against unbalanced genotypes, which could be tracked using markers for chromosome 1U. In wheat F4–F6 hybrids from crosses with Ae. biuncialis, we revealed a high frequency of formation of genotypes possessing the 1UL arm and lacking 1US. Conclusions. Since the Glu-U1 locus on the arm 1UL encodes high-molecular-weight subunits which directly determine bread-making quality, the developed wheat material is a source of a new allele of this locus introgressed from Ae. biuncialis for enriching the common wheat gene pool. Keywords: Triticum aestivum, Aegilops biuncialis, storage proteins, introgression.

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